FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_82.35100000039aa7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_82.35100000039aa7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177222
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGCC200.005137070529.9983089
TTCCACT350.002992320321.433414
CCTTATA455.433262E-420.0045152
TCTGAAC400.0064808318.7542343
TCATACT550.002089061816.3673324
GTATAGA705.990939E-415.0033871
GTATTAA850.002592850712.355731
TAAACAA850.002592850712.355735
GTATATA1006.389514E-412.0027091
TAATACA1202.3180316E-411.2525414
ATAATAC1100.001419660810.9115543
AAACAAT1000.00871339310.5023716
Produced by FastQC (version 0.11.2)