FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_81.35100000039a63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_81.35100000039a63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377683
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC350.003004075721.42658413
CGCTTGC400.00650607118.74826213
TTATACT1203.8653525E-916.2549514
GTCGCTA653.4314016E-416.1523511
TATACTG1401.8280844E-915.0045695
GTATAAC750.001013387514.0042651
TAAGGCT650.006362731613.8503714
CTGGAGT650.006362731613.8503714
TAAAGCT902.6377567E-413.3373954
GATAAGT800.001649036713.1289991
GTATAAA1101.0363698E-412.2764661
TCTATAC1354.172458E-612.2259453
CTATTAA1406.26116E-611.7893041
GTCTATA900.00398948111.670222
CAACACG900.00398948111.670224
ATTATAC1303.8156846E-511.5419763
ACTGTGT1059.691947E-411.4275128
GTTTATA1459.252706E-611.3827762
AGAAGTG1202.3295934E-411.25342755
TTTATAC1355.6065794E-511.1144963
Produced by FastQC (version 0.11.2)