FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_80.35100000039a20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_80.35100000039a20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258737
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGT200.0051338230.0073456
AATACCC300.00122223925.0061245
TCTACAC504.5763005E-521.0051443
TCTATAC608.193361E-620.0048983
AGGGGGG400.00648799818.7545915
CTATACT550.00209229616.3676454
GTACTGG859.12699E-615.8862421
CTACACT600.00373735915.00367454
CTCTTAT851.6223539E-414.1211031
GTGTAAA750.001012327514.0034281
AGTACAG750.001012327514.0034285
ATACAGA650.00635810213.8495456
AGCCCAC650.006374413613.84419219
CAGATAC650.006374413613.8441929
TACACAA954.1593588E-412.6346725
TATATAC1151.5657813E-411.7420053
ACTATGC900.00399698311.6650138
TATACAC1059.646579E-411.431375
ATATACA1202.3254841E-411.2527554
GTACAGT1000.00873038310.5025726
Produced by FastQC (version 0.11.2)