FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_79.351000000399eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_79.351000000399eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715745
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG755.2350515E-922.0038434
CCCCAGA559.580613E-519.0889111
CGTGAGC703.112568E-517.1410612
CTAATAC1950.016.156673
CCACGAG1052.4750807E-715.713737520
TAGACAG809.729472E-515.002625
TAGGACC600.003747719815.002624
GCTGGGT706.0345134E-414.99947617
CTCCGAG1251.1321208E-714.39849112
CCGAGCC1156.941318E-714.34632314
CGAGCCC1156.941318E-714.34632315
TACAGGC1054.268966E-614.2842177
ATAGGGG952.6280624E-514.2130093
AGGGGGG1402.9755029E-813.93100555
CCCCCAG650.00638737913.84470310
CGCCAAG650.00638737913.84470314
GCTCACT650.00638737913.84470312
CCTAATA2400.013.7524012
ACTATGC2550.013.5279948
TCCGAGC1352.8950308E-713.33193713
Produced by FastQC (version 0.11.2)