FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_78.351000000399a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_78.351000000399a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360542
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG2000.016.5014674
TAGGTAG2300.013.0446395
TGGGGGG2950.012.7129965
GCCGCGT954.1871794E-412.63270325
CCTACAC850.002611925612.354043
AGGGGGG3400.011.9128255
GGGACGG1406.3274783E-611.7847659
CTAATAC6900.011.3053533
GTCTTAG3100.011.1300231
AGTCCCG1000.00878692410.49915510
CGGGGGC1150.002077167610.43416317
CGGTCGG1150.002077533410.43394313
TTGGGGG2752.1645974E-1010.3645584
CTAGGAG1901.5779005E-610.26407053
TCTATAC5900.010.1703973
GTAGGGG1809.246165E-610.000893
TATACTG8550.010.000895
TACACCG2105.248585E-710.000895
GCTCACG1809.264821E-69.99919512
GGTATAT7700.09.9359481
Produced by FastQC (version 0.11.2)