FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_77.35100000039964.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_77.35100000039964.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637033
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG3150.018.0992015
GCGCACT952.6386359E-514.20929611
TTGGGGG2700.013.891934
GTATTAG3400.013.6790481
AGGGGGG2101.8189894E-1213.5744015
AGACGGG900.004001775811.6692216
CTAATAC4050.011.48364453
GTCCTAA1807.85858E-710.8353741
CTAGGAC1150.002072309410.43674853
TAGGGGG1451.1645176E-410.3470934
TTATACT6650.09.9269854
TATACGG5000.09.9021685
ACGGTGT3357.2759576E-129.8501958
GTATTAT8700.09.8294371
TAGACAG2609.84619E-99.8098395
ATTATAC8200.09.6969483
TACGGTG3552.7284841E-119.2952547
GCTGTAC1300.0057249899.2302518
CTATGCA3501.9463187E-108.99949559
GTGTAAT4201.8189894E-128.9302541
Produced by FastQC (version 0.11.2)