FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_76.3510000003993e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_76.3510000003993e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470213
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT200.00514473629.99936122
GGTTAGG350.003002061321.4303951
ACAGATC500.00110905617.9996178
TATTAGC750.001015721514.0011912
GTATTAG1402.9704097E-813.9297561
TTAGCTC650.006375058613.8473324
GTCTTAA800.001652814913.1261171
ACTATGC1056.734628E-512.856878
GGTCTAA850.00260663912.3539921
CTATGCA1252.5666124E-511.9997449
GTATAGA1151.5733538E-411.7401291
GTGCTAT900.00399852311.667661
GGATTAT1202.3366643E-411.25095751
CCAGAAT950.005983889611.0535723
TAGATAG950.005983889611.0535725
GTTATAT2351.8535502E-910.8519871
GGTATAT1253.41054E-410.8009191
GTATATG2104.526919E-810.7151981
ATACTGT1551.9453068E-510.6460676
CAGAAGT1000.00875861510.5008934
Produced by FastQC (version 0.11.2)