FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_75.351000000398f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_75.351000000398f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148581
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG4550.016.155245
AGGGGGG3950.015.5709635
TAGGGGG3100.015.4852084
TTAGGGG1905.4569682E-1113.4222113
CCGTCCA1059.7248954E-411.4277449
CTGCGCA1355.6586345E-511.1103069
TTACGGC950.005996679411.0535864
CCCCTAG1000.008777156510.5009071
CCGTGTG1150.002077726410.4340269
CCCAGAG1756.613529E-610.2866032
TAGACGG1501.6436083E-410.0008625
GACCGTG1200.00295807179.9992757
GTTCTAA5050.09.8028261
CAGGCAG2505.6466888E-89.60052428
TCCAGGA3306.002665E-119.5462792
CGAGCCC2209.93512E-79.54491315
CCCAGCC1450.0013404869.309678
TATTATA18200.09.2315652
GTATATA20650.09.0080411
GGACTGT2505.7927537E-79.0007766
Produced by FastQC (version 0.11.2)