FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_74.351000000398b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_74.351000000398b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473389
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGCA400.0065134318.7489629
TGGGGGG1850.017.0272395
TAGGGGG1551.8189894E-1216.4518184
TTCGTGC550.002100417416.36550725
CGGGGGG550.00210184716.363845
AGGGGGG2300.015.0001865
GGCACAC650.006388084513.84585722
AAGACCG1006.450195E-412.000155
AGTACCG1151.5787952E-411.7392765
ATAGGGG1551.5437436E-611.6130493
TAGACTG1202.3446916E-411.250145
CCAGGAG1150.002075132210.4349123
CTTAGAC1602.7749373E-510.3126293
TACACTG1602.7749373E-510.3126295
GTATTAG5100.010.0001251
TTGGGGG2105.248312E-710.0001254
TGGACTG1653.893568E-510.0001245
GTACGTG1200.00295606129.9994469
CCTATGG1250.0041409999.600122
TTATACA6750.09.55567554
Produced by FastQC (version 0.11.2)