FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_73.35100000039872.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_73.35100000039872.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719290
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAT500.001108052618.0039221
GGACCGT600.003744933815.0043126
TCCTATG809.7259996E-515.0032692
TATAGGC750.001015620514.0030513
GGTATAT1004.257427E-513.5029421
GGTTATA1004.257427E-513.5029421
GTTATAG1004.257427E-513.5029421
AGTGTGA902.651136E-413.332538
TATATCT954.1739029E-412.6352094
GTATAAG954.1763522E-412.6343311
TGTAGTG1456.859718E-712.4173625
TAAGGCG1456.8770714E-712.41477229
GTTTATG850.002606357712.3556331
GTACTGC1354.188114E-612.2257356
AGACTAA1901.0400981E-811.84139125
TACACAT2052.5393092E-911.7106835
TCAGGAT900.00399807711.6692093
GTGCAAC900.00400847611.66515311
TAATGGT1059.6797344E-411.4318574
ATATATA1602.2509666E-611.2524521
Produced by FastQC (version 0.11.2)