FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_72.3510000003983f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_72.3510000003983f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72
Sequences flagged as poor quality0
Sequence length36
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGCGCTCTGAGATAACAGAGATCGACAAAACATG11.3888888888888888No Hit
ACGCAGCAATGGTACACTACAGTTGACTTTCAAAGA11.3888888888888888No Hit
ATTCCTTACAAGCGCTGAAAAAATGAAGAGATGATT11.3888888888888888No Hit
ATCTCTATTCTACCTGTCATCCCTATATAACGTCAT11.3888888888888888No Hit
CCACTGTCTAGAGCCTGATACGGAGGCAAATTGGGA11.3888888888888888No Hit
AACTTAAAACGTATTGATTGAACGCCCGTATCATAC11.3888888888888888No Hit
GATTTTAGAAATATTTTTAATCCACACTCGCAACAA11.3888888888888888No Hit
AACTAAACGTACCTTTGAACCCAAAAGGAAAGAAAC11.3888888888888888No Hit
TTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT11.3888888888888888No Hit
CGACAACTATGTGAAGCACATGGGGCGAGAATATCA11.3888888888888888No Hit
ATTATCATTGTCCACTCAGTCAACCAAAGTTCTGGC11.3888888888888888No Hit
GGTATACACCCTCCACACTAATTCAAGTTCATCTTG11.3888888888888888No Hit
CCGCTAAGGAGTGTGTAACAACTCACCGGCCGAATG11.3888888888888888No Hit
TGAACTGCCTCTGCTTAAAGGAATAACGTGATTATT11.3888888888888888No Hit
CCTATGCAAAATCCCATAACTAACCTACCATTCGAT11.3888888888888888No Hit
GCCTCCGAAGAATGATGGAGTAGAAATCTCCGGCAT11.3888888888888888No Hit
GGTAAACAAGCGTAGAGCGGACAATGGACACGAGTA11.3888888888888888No Hit
TAACATCAGTGCCCGGCCCGTGCACCACACCCAGGC11.3888888888888888No Hit
CAAATACCGAAGGAGAGGCTGAGGCGATTAGGGGAG11.3888888888888888No Hit
CGTTGAATACTTTCCCCTGGAAGCTTTTGAGCAAGC11.3888888888888888No Hit
ATTCACAACGGCTCCTAGAGGCCCTAAGAAAGCGCA11.3888888888888888No Hit
ATCCACCACTGGCAAAATATTCACCACTAGAACCGC11.3888888888888888No Hit
ATCAATACTCGACTTCCTGGGAGAACACAACCGTTA11.3888888888888888No Hit
GTATAAATACTTGTACGTTCCTCTTGTAATTTCCCT11.3888888888888888No Hit
GGATAAATCACTCGAACATGCTGGTTTGCCTATACT11.3888888888888888No Hit
TCCTAAGGGGGGCTCATGGAGAACAGAAATCACCAG11.3888888888888888No Hit
GAACACATAACAACTCATCATACCGATGCTGTTTTA11.3888888888888888No Hit
GATCCACACACCCGAAGTCAAATTAATAATTGTATT11.3888888888888888No Hit
ACATAAATACAAAAGTACCAAAAGATGGCGAGGCTT11.3888888888888888No Hit
CTTGTCAAGAAACGAACAAACCACTTTCATTTAAAT11.3888888888888888No Hit
GAAGAGCTTATCAAAGAGATGGCCATAACAGAAAGG11.3888888888888888No Hit
TATTCCATTTGAGATCACCGCAAACAACTACAAAAG11.3888888888888888No Hit
GAATATATATCAGTAATTGGTCAAAATCACTTTGAA11.3888888888888888No Hit
CCACAGAATTACGCCAGAAGAGGCTCTGTCAGAGAT11.3888888888888888No Hit
GGAGTAACTAAGACTCTCTTAAGGTAGCCAAATGCC11.3888888888888888No Hit
CCCTTAGAGAAGAATCGAGGGAATGCATAGGCAGAT11.3888888888888888No Hit
GGTGCAGAAAGTATTGCAGCAGTGAAGAGGACAGCG11.3888888888888888No Hit
GTATAATCCTTGGAAGTCATGATGGGAACCAATGAT11.3888888888888888No Hit
GGTCATTGATTTTGAGGTCAATGGTGACTGCTTCGG11.3888888888888888No Hit
CATGTACATAAGTGGGAGTACTAACTCCTCATCCAA11.3888888888888888No Hit
TGATAAAAATCATGTTCTTTATCTTGCTCGAAGTTT11.3888888888888888No Hit
GTCCTGACGTTGATGCTGGAAGTGGTAGTAGCATTA11.3888888888888888No Hit
CTCATTCTTTACCAAGTTACGCAATACCTCCAACGT11.3888888888888888No Hit
AAATGACCTTTATCCAGTTCTCTTTACACAAACTTA11.3888888888888888No Hit
TACTTATACACACGCCCAAACTTTCAACAACCATTC11.3888888888888888No Hit
GGGTTATATGGAGCTTCTGTGATCAACACCGGATGT11.3888888888888888No Hit
ATGCCCCTGTAGGTACCAGAGACACCAGAGACATCC11.3888888888888888No Hit
GTATAAAATTGTCCACGTAAGCTAGTTGTTCCAACA11.3888888888888888No Hit
CCCTACCAACGAAATAGGAAAAGAAAAGAAATGACT11.3888888888888888No Hit
ACAGTGTATGTATCGACGTTGAACCACATCAACCCC11.3888888888888888No Hit
AATGTGTGGAAATACTAGGAGGCGAGCTTATTGCTT11.3888888888888888No Hit
TTGCAAAACGTCCTCAAACAACAAGCTCTCAAAAGG11.3888888888888888No Hit
CTTTAATACCCTTCAAGACCAACTACTCCCTCAGTG11.3888888888888888No Hit
CCGTAATGTAGACCGACTCAGGTATGTAAGTAGAGA11.3888888888888888No Hit
AAAAATTTCAGGAAAGTGATGACTAGAAAAACGATG11.3888888888888888No Hit
CTCTCTGGAAGTTAAACTGTTTGGCTTGATAGGAAT11.3888888888888888No Hit
ATAAAGGACTAGTGTACGTTTTTTGTGTTTAATTAT11.3888888888888888No Hit
AGAACACATACACAGTAAACACGGCGACCAGAAGAA11.3888888888888888No Hit
AACTTGCTTGTTAAAAAGTATTTGGTCAGCCGTGCC11.3888888888888888No Hit
CCAATTAGCAAAACAAAAAAAAACACCCAGACACCG11.3888888888888888No Hit
GTGTCTTGTTGCACTTAATCTATCGCGATAGCGCCA11.3888888888888888No Hit
AACTCGGTCACCCGCACAGCAAGATACGCCCTGTTC11.3888888888888888No Hit
ACACAATAGCAATCGATGATTCTAAAGCTCTCTTTG11.3888888888888888No Hit
GTGCTCCACTGGCTGTTTCGCCGGATCGGCGGGCCC11.3888888888888888No Hit
GGTGAAGGCATCGAATATAAGCCCCTTAACAAGAGT11.3888888888888888No Hit
TCTTTATATCGTCTGCTTGCGTATAATCTAGGCCAC11.3888888888888888No Hit
AAAAAGAACGAGACCTAGGCAAAAGTCAACTTCAAA11.3888888888888888No Hit
ATGTCCGGACAGACTTCAAGAATTGAATTGTTATTA11.3888888888888888No Hit
CTCAAACAGGGTCCGCTGCATGGTCGTGCGGTCGTA11.3888888888888888No Hit
GTATACAAGACTATTGAATTTCACAGGCGAAACGAG11.3888888888888888No Hit
ATATATGAGTAATTGTTTATATGCAATAGCGTTTGA11.3888888888888888No Hit
TTCAATAGACTTCAGTCACGGATCCTTGGTCTAGAG11.3888888888888888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGT50.029.9999981
GTATGTA50.029.9999986
GTTGAAC50.029.99999818
CACATCA50.029.99999825
TCGACGT50.029.99999813
GTGTATG50.029.9999984
ACGTTGA50.029.99999816
ACCACAT50.029.99999823
CAACCCC50.029.99999830
TATGTAT50.029.9999987
TATCGAC50.029.99999811
GTATCGA50.029.99999810
TCAACCC50.029.99999829
CATCAAC50.029.99999827
ACATCAA50.029.99999826
AACCACA50.029.99999822
GACGTTG50.029.99999815
AGTGTAT50.029.9999983
GAACCAC50.029.99999821
CCACATC50.029.99999824
Produced by FastQC (version 0.11.2)