FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_71.35100000039808.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_71.35100000039808.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77144
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCA1150.14907186560199107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT200.00509837830.0233784
ATAAATA504.5043183E-521.0163632
GTATAAG400.006476205318.74027312
AAGAGAC550.002085031216.35514616
ATAAGAG550.002085031216.35514614
TAAGAGA653.3983216E-416.14546415
AGAGACA600.003724626314.99221817
Produced by FastQC (version 0.11.2)