FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_70.351000000397c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_70.351000000397c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575263
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG559.571518E-519.090195
AGGGGGG500.001109587617.9993235
ATAATAG1203.9017323E-916.2479763
CTGTGCA755.6148423E-515.9993979
GCATTAA706.0320855E-414.9994351
GGACAGA600.003751824614.9994356
TAATAGC600.00375399514.9981324
CATAATA1004.2682455E-513.4983182
TATACTG954.1831008E-412.63110355
TAATAGT954.1861695E-412.6300054
GTGTAGA1151.5763263E-411.7386881
AAACAGC1151.5763263E-411.7386886
TATAATA1355.6430297E-511.1097272
TATTCTG950.00599211811.0522165
ACCGGTC1408.159961E-510.7138828
GGAGAGC1408.159961E-510.7138826
TATAGTG1408.159961E-510.7138825
TAGAACA1000.00877624110.4986924
TTTGAGG1304.9001677E-410.38602925
CAACACA1602.7710797E-510.3112154
Produced by FastQC (version 0.11.2)