FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_67.35100000039709.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_67.35100000039709.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849122
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCA550.002102716816.3616779
CCTAGAC653.4338035E-416.1547663
AGGGGGG901.5797326E-515.00085455
GGCTATA809.7426484E-515.00085451
TTACACC1251.1307384E-714.4008194
TAGGGGG952.6321219E-514.2113364
GGACTTA851.6318617E-414.118456
GAGTGTG851.6343126E-414.1159577
CCAACGC650.00637934113.84775722
GTAGGAC954.1834055E-412.6322983
CTTACAC1201.6910326E-512.5007123
CCTATAA1406.304441E-611.7863861
ACATAGA1151.5766319E-411.7397991
GTTCTAC1806.2455E-811.6673321
CTCTTAT1806.2455E-811.6673321
TAGACCG1059.7015954E-411.4292225
TGTACAG1059.7015954E-411.4292225
TAACCTG1602.256902E-611.2506415
GACCTAA1355.6399316E-511.1117441
ATACCGT2301.3078534E-911.0875876
Produced by FastQC (version 0.11.2)