FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_66.351000000396c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_66.351000000396c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91799
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATG1750.19063388490070698No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCA1000.1089336485146897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT400.00645444518.7557951
CTTTCCA400.00645444518.75579524
AGGCATG400.00647481618.74557330
TCAAGAA750.001003110414.00432622
Produced by FastQC (version 0.11.2)