FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_65.3510000003969e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_65.3510000003969e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890931
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG1251.13259375E-714.4014984
TAGACAG2350.012.7672865
GTCCTAT1704.676085E-610.5890561
GTATTAG2301.5485057E-810.4355931
GATTAGA2453.7234713E-910.4089711
TCCTATA3203.6379788E-1210.31332
ACTGCGC1756.6222237E-610.2848818
GTCATAT2752.3665052E-99.8189431
CTGACCA2454.1365638E-89.7951249
AGGGGGG2003.0397568E-69.7510155
TTAGGAC1250.00413969079.6007443
GTAGGGG1250.00413969079.6007443
TGGGGGG2557.648305E-89.4127445
GTATATA5900.09.407511
GTCTAAG1450.00133813239.3110681
CCTTATA2752.4665496E-89.2734462
TTAGACT1952.3855637E-59.2317294
GTATAGG2301.8138144E-69.1311441
TATACTG3951.8189894E-129.1148735
CCTATAG1654.2799255E-49.0916153
Produced by FastQC (version 0.11.2)