FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_64.3510000003964e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_64.3510000003964e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214948
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGCG901.9663275E-919.99832724
GCCGCGT1007.2705006E-917.99923525
TAGGGGG1306.475602E-1016.1564854
TAGGTAG1501.8189894E-1116.0026135
TCGCCGC1256.719347E-915.59869423
TAAGACT1301.12449925E-815.0024494
TAGTCCC851.6335852E-414.11762828
GGGGGCT750.00101801513.999989
GGGGCTC750.00101801513.9999810
GCGCACT750.001018331713.99940411
TGGGGGG1907.712515E-1012.6336425
ACGGTGT1907.730705E-1012.6315628
ACCGCGT1201.6941818E-512.49946817
GCTAGAC850.002610018212.354453
GGGGGGC850.00261243712.3529248
ACTCCGC1703.7617792E-711.4705728
GGTAGTC1459.335899E-611.3788267
TATACTG3650.011.0977035
GTATTAG3250.011.0787321
TATACGG2751.8189894E-1110.9108725
Produced by FastQC (version 0.11.2)