FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_63.3510000003960a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_63.3510000003960a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853036
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG1450.019.659014
TGGGGGG3200.019.2225025
CTAATAC4400.014.3209763
TTGGGGG2950.013.7314934
AGGGGGG2850.013.6868825
TGCGCGG954.1922077E-412.63063610
GACCGCG850.002614767312.3520187
GACGCGC850.002614767312.3520188
CCTAATA5300.011.6060392
GTGTAGG1059.699823E-411.4308021
CCGTCGC1859.174255E-811.3505039
GACTGAT1355.6558507E-511.1102817
TCTAGAC1901.3218414E-711.054793
GTGACGC950.00599024411.054796
TAGACTA1602.7704074E-510.3145135
ATAGGGG2209.0096364E-810.2292693
TAGACCG2957.2759576E-1110.1714765
CAGGCCC1356.964193E-49.9992539
TACTATG5000.09.8992617
TAATACT8350.09.8821694
Produced by FastQC (version 0.11.2)