FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_62.351000000395c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_62.351000000395c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067811
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGC1102.3495886E-816.3680823
CTTACAC1054.25707E-614.2895953
GGACTGT650.00637219613.8505656
TAGGGGG650.00637417413.8499164
TCTAAGC1201.1186166E-613.7537363
GACTTAG1106.9156486E-613.6349587
TGGGGGG1454.7146386E-813.4525595
GTATTAG2500.013.2035881
TAGAACT2102.1827873E-1112.8606354
GCCTAGA1056.731734E-512.8606351
GGCGCCG1056.749483E-512.85702125
ACTGTGC954.190627E-412.6302788
TAGGTAG1551.12737325E-712.5846535
GCGCCGA1201.69315E-512.49988426
AGGGGGG1101.0396671E-412.2766365
GTACAGG1252.5648518E-512.0038226
GCGTAGA1006.433597E-412.0032611
GACTAGG900.004009850311.6654648
CGCCGAA1303.8432594E-511.53835427
TAATACT4250.011.2971864
Produced by FastQC (version 0.11.2)