FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_61.35100000039582.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_61.35100000039582.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492896
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2500.020.4032345
CCCCGAC400.006512981418.74920516
TAGGGGG1002.5705103E-615.0023794
AGGGGGG1900.015.0023795
CCCCCGA600.003755680814.99936415
TTGGGGG1751.2732926E-1114.5737394
GACCGCT650.006390439313.8451027
GAGCGCG850.002612410812.35324629
AGACCGC1101.0417574E-412.2746736
ACTATGC4400.012.2717958
CTAGGGT1006.442366E-412.0019044
CTAATAC4350.011.7259973
TAATACT6350.011.5766384
TATGGGG2301.3096724E-911.0887153
TCGGGTC950.00599992111.05216224
GATCGGT950.006001416611.05179211
GACAGTC1100.001434025710.9082627
TACTATG4800.010.6241937
CTATGCC2301.5510523E-810.43399059
CCTACAC1356.947598E-410.0015863
Produced by FastQC (version 0.11.2)