FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_60.3510000003954f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_60.3510000003954f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675388
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGTCG400.006508984618.749422
TAGCCCT500.001109817817.9994249
TGGGGGG1202.0736479E-1017.5007345
TAGGTAG706.030372E-415.0006285
ATTACTC1004.26372E-513.50056653
GTCTATA1651.8566425E-812.7278061
CTTACAC850.002609515312.3534593
CAATGCG850.002610965212.3525459
CTATGCA2001.7298589E-911.9996169
ATTGTGC900.004005118311.6662938
CTCTTAT2103.7198333E-911.429051
AAGGAGC1059.698319E-411.429056
ACTGATC1059.704336E-411.4282058
CTAATAC1859.121504E-811.3518283
TTGGGGG1202.3404753E-411.2504724
GTCCAGA950.005990396711.0530961
AATACGC950.005990396711.0530965
ATTGACC950.005993698711.0522778
GTATTAG2104.544563E-810.7147351
TACCCCT1000.00876806210.5004415
Produced by FastQC (version 0.11.2)