FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_59.3510000003950b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_59.3510000003950b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151044
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCC800.001657421513.1239369
GGACAGC1151.093374E-513.0429876
TGGGGGG1551.1329212E-712.5801735
AGACGGC850.00261300312.3524766
GTAACCC1006.4510223E-411.9995483
CCCAGCC1006.4533786E-411.99902613
CTTGGGC1151.5789561E-411.7386883
AGAGTGC900.00400821711.6662276
ACTCCGC900.004009518311.6657218
ACTGGCC1303.8466867E-511.5375258
GTCTAAG1602.2617642E-611.2495771
TTGGGGG1355.6494115E-511.1106924
GGACCCT1100.001433135710.908686
AGGGGGG1807.8737685E-710.8329255
GCGCCGA1000.0087682410.5014326
TAGACGG1000.00877954310.4996045
AACCGTG1000.00878237210.4991487
AGACTGG1150.002075403210.4343896
ATAGGGG1150.002075403210.4343893
GTTCTAG1304.916041E-410.3842241
Produced by FastQC (version 0.11.2)