FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_58.351000000394c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_58.351000000394c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732132
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG908.557254E-716.66945
TTGGGGG706.025646E-415.00246054
GCTCTAG600.003748096315.002461
GGGTAGG650.00637620213.8484231
CCAGAAC1004.2597916E-513.50221353
GTATTAG1651.8544597E-812.729361
TACACCG954.1784102E-412.6336515
TCTAAGC1006.435167E-412.0019673
GTCTTAG1006.435167E-412.0019671
CACAGTG1006.4462615E-411.9995087
CCAGCAC1151.5744947E-411.7410553
ACACTGT1151.5744947E-411.7410556
ATTAGGT1806.230948E-811.6685793
ATTAGGG900.003999806511.6685793
TGTAGAC900.003999806511.6685793
GTGTACG900.004005935511.6661899
ATTAGAC1303.8326252E-511.5403533
TATTAGG2351.5279511E-1011.4912452
CTAACAC1202.338337E-411.2518453
TTATCCT2155.3714757E-911.164624
Produced by FastQC (version 0.11.2)