FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_57.35100000039483.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_57.35100000039483.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520410
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC359.967368E-525.713463
TGGGGGG608.239285E-619.9993615
GGACTGG500.001109351617.9994266
TAGGTAG752.7910737E-617.9994225
GTATTAG1451.0913936E-1116.5511931
CCCTTAT1203.8944563E-916.2494811
CTATGCA1203.8980943E-916.247929
ATACAGG1351.1023076E-915.5550586
CCTTATA1503.0377123E-1014.9995192
ATAGGGG706.0304604E-414.9995193
TCCAGTA901.5787278E-514.9995192
ACTATGC1206.857044E-814.9980798
GGTAGTC809.746484E-514.9980787
AGGGGGG851.6311782E-414.1171965
TAAGAGT851.6311782E-414.1171964
GACAGCT650.00638527613.8443797
TTAGGTA1201.1192769E-613.749564
AGTGAGT1004.2668522E-513.4982718
GTATTAA1352.8873546E-713.3329071
GTCCTAT902.648298E-413.3329071
Produced by FastQC (version 0.11.2)