FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_56.35100000039440.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_56.35100000039440.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781511
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC1102.355955E-816.3628713
CTCCGAG1104.1970088E-714.99929612
CCGAGCC1054.264786E-614.28595914
TAGGTAG650.00638021113.8472775
CGAGCCC1201.1207267E-613.75023515
TAAAGTG2102.1827873E-1112.8581865
ACCATGC1056.747351E-512.856548
TATACCC1354.199097E-612.2232125
GTATAAT1856.9921953E-912.1631481
CGAGACC1252.572399E-511.99943723
ACAGTGT1406.3099396E-611.7851628
AGACTGT1151.5759711E-411.7400826
GTTCAGA1151.5759711E-411.7400821
CTTACGC900.00400269711.6676123
GCCCACG1303.839151E-511.53865818
ACGAGAC1303.8416605E-511.5379222
CCTTATA1859.1245965E-811.3522722
CTATCCT1602.255494E-611.2509134
TAGATAG1602.255494E-611.2509135
CAGAACT1202.3405219E-411.2509124
Produced by FastQC (version 0.11.2)