FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_55.3510000003940c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_55.3510000003940c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062750
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG408.174044E-626.2569181
CCGTGCC400.006514426318.7478839
TTCGGCG706.037191E-414.99971830
ACTGCGT600.003756494714.9983058
AGACAGT1554.965841E-1014.5199566
TAAGACA2000.014.2537564
CTCGTCC650.00639040813.8445919
CCACCTG650.00639040813.8445919
ACCTGTG1201.1234606E-613.7484467
TTGGGGG1106.889426E-613.6399574
AATACTA3500.013.2892155
CCTAGCG800.001656499213.12475330
CACCTAG800.001656499213.12475328
GTATTAG2750.013.0943581
TCACACA2650.013.01862430
ATCACAC2700.012.77753729
CTATGCA2700.012.7763359
CGTTCAG1302.7613205E-612.69087510
TAATACT5100.012.6503934
GGGCACC850.002614251812.35154611
Produced by FastQC (version 0.11.2)