FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_54.351000000393d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_54.351000000393d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366191
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG805.1650513E-616.8782544
AGTGCCC951.5099358E-615.789058
TGGGGGG1608.021743E-1014.0652125
TGGCGAC902.6522984E-413.33346430
TCTAAGC2051.4551915E-1113.172793
GTCCTAA1302.7546394E-612.694291
GCCTCAC1201.6949107E-512.49920720
CTGTCCG850.00261322212.352619
CTGGGCG850.00261322212.352619
TTGGGGG1754.222602E-812.0023154
TACACCG1252.5693926E-512.0023135
ACTATGC3300.011.8178658
TCTACAC2553.6379788E-1211.7665433
CTAAGCA2052.5538611E-911.7095754
GAACCGC900.004001975511.6689166
ACTGTCC1703.764344E-711.4702818
CTAATAC4100.011.3432353
AGGGGGG1602.2565218E-611.252175
CTAGCAC2002.2206223E-811.2517573
ACCTGTG1202.3453089E-411.2496987
Produced by FastQC (version 0.11.2)