FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_53.35100000039391.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_53.35100000039391.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501898
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATATACATGAGAAGAACGGCATAGTGCG8710.173541237462592No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACG7390.14724107288731975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAC359.9496596E-525.7199252
TAGGGGG402.461166E-422.5049344
AGCGCAC553.8805465E-621.81644410
CCCACAT400.006504753618.75037427
TCTAAGC500.00110740818.0039463
GCGCACA601.8641709E-417.49860411
GTCTAGG550.002096349116.3672261
TAGGTAG653.4260633E-416.1573895
AGGGGGG706.0184376E-415.0032895
TAGTCAG600.003751985314.9988049
GTATTAG1251.1253542E-714.4031581
TGGGGGG851.6275166E-414.1207435
AATTCGG750.001017155213.99888320
TAGAGCG750.001017921813.9974897
GCACCAC650.0063701813.849196
TAAACAG1004.252351E-513.502965
TATACCG1004.252351E-513.502965
GTATTAA1454.701178E-813.4512241
TGAACAC902.642375E-413.3362575
AGCACAT800.001651414713.1278785
Produced by FastQC (version 0.11.2)