FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_52.3510000003935e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_52.3510000003935e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955933
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCC951.5101668E-615.7876558
CAGTCCC600.003756180914.9982739
TATACCG1751.2732926E-1114.5735635
GTCTAGA1351.8477294E-814.4465611
AGGGGGG1251.1300108E-714.402115
GACACGA650.006389881513.84456112
GCAGGCC650.006389881513.8445618
AACGACC800.001657487713.123497
AGACCGT1601.7078673E-712.1892856
CTCCTAA1151.5757157E-411.740851
ACCGTCG1151.5795574E-411.737788
TAGGGGG900.004001857711.6683764
GAACGAC900.004001857711.6683766
CCGTCGC900.004009686411.6653249
TAGGTAG1303.836168E-511.5401525
TCTAACC1303.836168E-511.5401523
ACTGAGC1059.717539E-411.4272568
GACCGTC1059.717539E-411.4272567
TATACTG4600.011.4147165
TAGACCG1459.310512E-611.3809785
Produced by FastQC (version 0.11.2)