FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_51.3510000003930d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_51.3510000003930d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584103
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG1850.020.2757115
CAGCGTG1352.9027433E-713.3318619
TTGGGGG2203.6379788E-1212.9580224
ACAACGC1302.7636743E-612.6909068
CCGTCTC850.002615180612.3515769
AGGACGG1006.4368057E-412.00322155
TAATACT8100.011.8550334
ACGGTGT3100.011.611628
ATACAGC3700.011.3543986
ACCGTAC1602.265513E-611.2487568
ACAGCGT2207.761628E-910.9078868
AGGGGGG2104.5487468E-810.7171615
TAGACAG2556.366463E-1010.5910775
AGCGCAC1704.6850528E-610.58706510
GGACAGT1851.1169195E-610.5433696
CTCCTAG1000.00876209110.5028191
CCCCTAT1150.00207086810.4375831
GGCTTAT1304.9043726E-410.3874031
TATACTG6950.010.3624935
TAGGGGG1451.1638813E-410.3476044
Produced by FastQC (version 0.11.2)