FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_50.351000000392c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_50.351000000392c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924549
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGGG400.006502646518.7534483
CTAGGAC550.002098644916.3666463
TCTAGGT706.0268573E-415.0027593
GTTCGCT706.03924E-414.9987029
TAGACGG1056.7353656E-512.85950855
GTATTAA2255.456968E-1212.6689971
ACGGTGT2051.9281288E-1012.4379488
AGGGGGG850.002607993312.3552145
ACTATGC2401.8189894E-1111.8739738
TGGGGGG1151.5750597E-411.7412895
TAGACAC900.004000569711.6688125
GGGAGGT900.004008662411.66565712
GAGAGGG1459.333731E-611.3783257
GTATTAG2402.2009772E-1011.2520691
CTAATAC3500.011.1449073
CTACACA1355.633898E-511.1131544
GTTGGAC950.00598690211.0546653
TAGTACG950.00598690211.0546654
CTGGACA1100.001430026310.9110974
ACATGCC1100.001433258110.9081478
Produced by FastQC (version 0.11.2)