FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_49.35100000039285.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_49.35100000039285.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885905
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG1851.8189894E-1214.594624
TGGGGGG3150.013.8095485
GTATTAG4050.012.9629861
CTAGGGT1056.7541114E-512.8571654
CCTACAC1907.730705E-1012.6316013
TAGGTAG2401.8189894E-1212.5000225
CCCTACA1601.7145248E-712.1875212
CCCTATA2750.012.0000212
ATAGGGG2052.562956E-911.7073383
GCTCACG900.004009452611.66637823
AGGGGGG3300.011.3636565
CCCCTAT1653.274099E-610.9091091
GTGTCCG1304.919778E-410.3840839
AGCACTG2752.1645974E-1010.3636545
GTATAGA4800.010.3125181
TTGGGGG3900.010.0000184
GACCTGC1501.644656E-49.99975223
GCAGGCC1501.645096E-49.9994888
CTAATAC4100.09.8780673
ACAGTGC2003.0457759E-69.7494998
Produced by FastQC (version 0.11.2)