FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_48.35100000039242.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_48.35100000039242.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289935
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGA2950.10174694328039043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC200.00514662429.99327515
CGGGCGT200.00514662429.99327513
CCATACA300.001221020525.0116484
GGAAGGC300.001225944624.9943948
GACGTAG603.2655953E-722.50271626
GTGGCGT350.002998692421.4311630
CGTAGAG657.0548595E-720.7717428
ATCCTAA608.183313E-620.009321
TAGAGGA701.4362977E-619.28804430
CCACGAG701.4410562E-619.2813920
CAGTAGG400.006507447518.74579614
CGGTGTT651.6421694E-518.45749
GAGACGT752.78295E-617.9990724
CTCCGAG752.7875394E-617.99596412
TCCGAGC752.7875394E-617.99596413
CCGAGCC752.7875394E-617.99596414
TAGTCAG601.862804E-417.4960779
GTATTAG703.0870848E-517.1508461
ACGGTGT703.1054362E-517.1390138
TTACTGC805.1234747E-616.8828666
Produced by FastQC (version 0.11.2)