FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_47.3510000003920e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_47.3510000003920e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586452
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1151.1277734E-1018.2608175
TAGGGGG1154.0634404E-815.6521294
CTTGTAC600.003751072814.9999573
CTATGCA809.746518E-514.9986789
ACTGATC809.746518E-514.9986788
TTCTAGG650.006381251413.8461152
CTGAGTA650.00638485713.84493359
ACTATGC1201.120683E-613.7487888
CTAATAC1507.356175E-812.9999633
TAGACAG1302.7519363E-612.6922725
ATAGGGG954.1820877E-412.6315433
CCTAGAA954.1820877E-412.6315432
GTTCAGG954.1820877E-412.6315431
CTCTTAT1201.6899641E-512.4999651
GTATAGG1406.299313E-611.7856811
TGTACTC900.004003169511.6666343
TACACTG1059.6986274E-411.4285395
GTATAGA1602.2546137E-611.2499681
TAGGTAG1100.001430902410.90906055
GTATAAA2751.8189894E-1110.909061
Produced by FastQC (version 0.11.2)