FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_46.351000000391c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_46.351000000391c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399384
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG300.001224437625.0025044
ACAGCGT350.003003195221.4280368
TAGACAG752.7842561E-618.0018045
ACTGGTC500.001108685717.9995488
TGGGGGG601.8604068E-417.5017535
ACTGCCT550.002098765216.3632268
TACAGCG653.4302523E-416.1534427
TTAGACT755.6013367E-516.0016044
CCCTGAT706.020061E-415.0015032
GTATTAG809.718515E-515.0015031
GTATAGA901.5749307E-515.0015021
ATACAGC1104.1751264E-715.0015026
GGATAAG650.006372203613.847541
TATACCC650.006372203613.847544
ATGGTTA650.00637749213.8458078
CTGCCTT1004.2578602E-513.4996619
ATTAGAC800.001651867813.1263153
AGTACAG800.001651867813.1263155
TCTATAC1551.1236807E-712.5819053
GAGTATA1006.427328E-412.0012031
Produced by FastQC (version 0.11.2)