FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_45.35100000039186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_45.35100000039186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271260
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCC200.00513778230.0031345
TAGGTAG504.58231E-521.0021955
TGGGGGG504.58231E-521.0021955
TAGTCAG455.447947E-420.002099
ATTAGAC559.537376E-519.0929033
TACCCTG400.006494056418.7519595
AACTCCC400.006500974318.74850326
TTAGACA550.00209461516.3653454
ACTACAC550.00209461516.3653453
GGTAGTC550.002097197816.362337
GAATAAC653.4217996E-416.1555331
CTATACT859.142443E-615.8840124
TAGACAA600.00374147815.0015685
ACCGTTC600.00374147815.0015688
GTATTAC706.011039E-415.0015671
TATACTG1301.1124939E-815.0015675
ATATACC851.6247503E-414.1191224
TCTATAC851.6247503E-414.1191223
GCATTAT902.6379316E-413.3347261
TTATAGT1056.7063076E-512.8584854
Produced by FastQC (version 0.11.2)