FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_44.35100000039143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_44.35100000039143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430708
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA300.001224645925.0023231
GCAAGCG350.003003542321.42807425
ACCGATG400.00650491418.7495658
TGGGGGG752.7854803E-618.0016735
TCATATA1501.8189894E-1116.0014882
AGACGGC600.003746530515.0013936
AATTAGG600.003746530515.0013932
TATGTAC706.021756E-415.0013933
TCGAGCA600.00374942614.99965223
TATACTG2200.014.3195115
GGATATA851.6284197E-414.1189571
ACAGTGC851.6300268E-414.1173198
GCAGATC851.6300268E-414.1173198
GTACTAA750.001015417914.0013011
TAGACGG650.006373583313.847445
GCATTGC650.006378487713.84583213
CCCTATA800.001652325713.1262192
CAAGGGT800.001652325713.1262194
CTACACA800.001652325713.1262194
CCCTTAT1151.088136E-513.0446891
Produced by FastQC (version 0.11.2)