FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_43.3510000003910f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_43.3510000003910f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331296
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT802.4782094E-718.7536811
GCCCACG400.0065049118.74801819
CGGGCGG400.0065049118.74801811
GCAGCGG400.0065049118.7480188
GTCTAGA500.001106497618.0035341
AACAGTC500.001108742617.9980987
TACTGTA550.002094646716.3668485
TCGCTTG653.4301498E-416.15213812
TAGACCG859.1467045E-615.8854715
TATACTG901.5718882E-515.0029445
AACGATC600.003745283515.00067927
GACCGTC706.0256233E-414.9984167
ACTGGGC706.0256233E-414.9984168
CCGTCGC809.7276075E-514.9984159
ACCGTCG809.7276075E-514.9984158
CGAGCCC851.629373E-414.11615516
TCTGTAC650.00636512313.8488713
TATTCCG650.00636512313.8488715
TAAGGTA650.00636512313.8488715
CGATCAA650.00636512313.84887129
Produced by FastQC (version 0.11.2)