FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_42.351000000390ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_42.351000000390ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912466
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG859.622454E-1021.1782475
GGGCAGG601.8662916E-417.49859412
TAGGGGG703.1090418E-517.1442994
ACTGCGC809.7553275E-514.9987948
TGGACTG902.6491203E-413.3344555
TAGCCAG800.001654904313.1261025
GGTAGTC1354.2088614E-612.221247
TGCGACG1006.436235E-412.00232530
GCTAAAC1303.8381688E-511.5394323
GTATTAG2351.5279511E-1011.4903281
CTAAACT2103.7252903E-911.4295324
TAGACAG1459.31568E-611.3802675
ATGGACT1202.341274E-411.2509454
AGAGCGA950.005998585411.051743510
CACGCAG1100.001433152410.90821516
TAGGTAG1408.1619495E-510.7151865
GTGCGAC1000.00876216110.50203429
TAAGCCT1000.00876928510.5008834
TACACCG1000.00876928510.5008835
GACCATG1000.0087799810.4991578
Produced by FastQC (version 0.11.2)