FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_41.35100000039087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_41.35100000039087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743732
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1051.3231329E-817.1459315
GTCCTAA951.5033875E-615.7933681
TTCTAGG1351.1004886E-915.5583452
GTCTAGC809.7243304E-515.0036981
TAGACTG600.003747787815.002695
TGGGGGG1206.844857E-815.002695
CAATCGG600.003754500514.99865513
CTTCTAG1402.97332E-813.9320061
CCTTAGT650.006372836413.8495681
TAGGACC902.645955E-413.3357244
TATAGGG1056.7322115E-512.8594492
ACTGATC954.1874964E-412.6304478
GGTTATA1201.687076E-512.5030821
TCTAGGG850.002607256212.3551573
GTTCTGG1354.190568E-612.2252361
TTGGGGG1252.5660509E-512.0021524
TCCTAAG1151.5743327E-411.7412362
GTGTATG900.003997439511.6695431
GGACAGT900.00399944911.6687586
TGTGCTG1059.6891786E-411.4306215
Produced by FastQC (version 0.11.2)