FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_40.35100000039044.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_40.35100000039044.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342632
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTC200.005143131629.998985
TAGGTAG400.006502666518.7493635
TGGGGGG500.001108315517.9993865
TGCGACG550.002098071416.36308130
GTATTAG755.604045E-515.99945451
AGTTATG755.604045E-515.999454530
ACTGAAT600.003747611314.9994891
TATACGG1206.825758E-814.9994895
ATAGGAT650.006375431513.8456823
GGTGCGA650.006375431513.84568228
GTGCGAC650.006375431513.84568229
GTTATAT1701.9717845E-913.2348431
ACAGCGA800.001652703613.1245548
CCTTATA954.1753068E-412.6311482
TACTATG850.00260647612.352527
ATACAGT1006.430472E-411.9995916
GTATAGA1151.5727103E-411.738731
ATAATAG1806.2051186E-811.666273
TACGGTG900.00399829411.666277
GGCAACA950.005983581311.05225611
Produced by FastQC (version 0.11.2)