FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_39.35100000038ff5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_39.35100000038ff5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631837
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG400.006505739418.7507926
GCAGTCC400.006505739418.75079217
TGGGGGG957.906419E-817.3691545
TAGGTAG908.563584E-716.6673725
GTCCAGA809.731019E-515.00182151
GAAGCGC650.006380066713.8467396
GTGCGAC1201.1184347E-613.75166929
CTAGAAC1004.262857E-513.5005693
TCCTGTA902.6480382E-413.3338972
AGTCCGA800.001656417613.12347710
TATACGG1907.694325E-1012.6321125
AACTGTG1201.6924212E-512.49854857
CTGTGGG850.002612289512.3515079
TAAGGAG1101.0410533E-412.2732465
GGTATAG1006.435318E-412.0014571
TGCGACG1406.2927556E-611.78714530
CAGCTAT1151.5744638E-411.7405551
GTCTTAA1151.5744638E-411.7405551
GTATTAG1806.2285835E-811.6680821
GTCCTAA900.004000039311.6680821
Produced by FastQC (version 0.11.2)