FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_38.35100000038fce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_38.35100000038fce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684375
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCC400.00650997318.748958
TAATACC1551.8189894E-1216.4555024
AGGACAG600.003745959415.0035465
GGACAGT750.001015390614.0033086
TTAGGGG650.00637258213.8494263
TTAATAC1752.0736479E-1013.7175263
ATAATAC2101.8189894E-1213.5746363
CTAATCC1151.0887557E-513.0465613
TATAAGG850.002605775612.3558612
TCTGAAC1101.03941195E-412.2756273
TCAGTAC1101.03941195E-412.2756273
TATAATA2257.094059E-1112.0028362
CTTAGAC1006.430308E-412.0028363
TCTATAC1406.286844E-611.7884993
GGAGGTC900.004005927611.6660148
TAAATCT1951.5204932E-811.5411884
TAATCCT1059.682828E-411.43127254
CTAAAAC1602.2499444E-611.2526583
TAGACAG950.00598187711.05524355
TCCAGGG950.00598187711.05524352
Produced by FastQC (version 0.11.2)