FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_37.35100000038f7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_37.35100000038f7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987929
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCT300.001226432725.09
TCCGAGC901.5811042E-514.99999913
TGGGGGG1851.8189894E-1214.5960715
CACGATG851.6330782E-414.11764714
AACCACG650.00638521113.8461537
CGAGCCC1006.447557E-412.015
TATACTC2800.011.7869085
GTCTAGT1602.2583026E-611.2505691
CTCCGAG1202.3434743E-411.2512
GATCGTT950.005995594411.0526317
CTGGACA1100.00143116410.9101954
CCTAATA1653.2670305E-610.9096432
GAGCCCA1653.2690132E-610.90909116
TAAGGCG1100.001432373810.90909129
CCCACGA1100.00143297910.90853819
CCACGAG1100.00143297910.90853820
CTATACT3450.010.8706664
CTGTGTG1253.42042E-410.7999999
GCTGTGT1000.00877561810.58
ATACCAT4300.010.4661756
Produced by FastQC (version 0.11.2)