FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_36.35100000038f3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_36.35100000038f3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497823
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAG400.00651096718.7472385
TGGGGGG1154.0663508E-815.6498695
TCTCCGA1002.570332E-614.99779111
TTGGGGG1054.264557E-614.2836094
ACAGGTG750.001017655113.9979375
TACAGGC650.006385573713.8441147
GCCCACG1106.893908E-613.63572518
TCCGAGC1151.0916445E-513.04155713
CGAGACG954.178124E-412.63225623
ACATCTC1907.694325E-1012.6297188
CCCACGA1201.6877719E-512.500669519
CTCCGAG1201.69126E-512.498157512
CGAGCCC1101.0410629E-412.27215315
ATCTCCG1101.04204024E-412.2709210
GTATAGG1101.04204024E-412.270921
AGACGTA1006.423892E-412.00305525
ACGAGAC1006.434754E-412.00064422
CACGAGA1252.5653531E-512.00064321
CCGAGCC1252.567995E-511.99943714
GTATTAG1252.5706391E-511.9982331
Produced by FastQC (version 0.11.2)