FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_35.35100000038f04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_35.35100000038f04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883741
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2600.016.732995
TAGGGGG1504.8949005E-914.0018594
AGGGGGG2250.013.3351035
CGAGCCC1006.455219E-411.99936415
CTATACT3800.011.84367754
TATTATA8250.010.5468542
GTATTAT7200.010.4180491
TTAGGGG1756.6053162E-610.287083
TATACTG4700.010.2141225
TCTATAC4500.010.00132753
GTTCTAG3152.3646862E-1110.0013271
GTCCTAA1809.238171E-610.0013271
TCGCACC1356.964621E-49.9992059
GTCTAAA3950.09.8747281
TAGGGAG1603.1430952E-49.3762455
GTATTAC3705.456968E-129.3255621
GTATTAG4050.09.26048851
GTACTAA3254.5474735E-109.2319951
ATTATAC7000.09.2155093
GTATTAA4450.09.1023311
Produced by FastQC (version 0.11.2)