FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_34.35100000038ecf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_34.35100000038ecf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799622
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT600.003751705215.0005324
AGGGGGG650.0063823113.8466445
AGACTGC850.00261036812.3533796
GTCGTAG850.00261036812.3533791
GTATTAG1652.549168E-711.8186011
GTACTAT2701.2732926E-1111.11150551
GTCTAAT1100.001431432810.9094771
CCTGTAC1100.001431432810.9094773
TCCTACA1807.854942E-710.8337172
GTATATG3700.010.5409141
TGGGGGG1000.00877085510.5003725
TTATGCT1150.002072950810.4351534
CTGTGGC1150.002072950810.4351539
TTACACT1451.1649776E-410.3451944
GTCTAAG1952.1955202E-610.0003551
CTTATAC1952.1955202E-610.0003553
TACTATA3305.456968E-1210.0003542
GTATATA5500.09.818531
GTTATAC1705.3935626E-59.7062261
AGGAGTT1552.2833701E-49.6777625
Produced by FastQC (version 0.11.2)