FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_33.35100000038e8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_33.35100000038e8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293119
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG350.003003449221.4254049
GTATATA1401.8244464E-915.0029011
TAAGGGT706.009029E-415.0029014
ACAGATG600.003748725414.9977838
AAGAGTC650.006377340313.8441077
TATACAG800.00164888313.1275395
CATATAC954.1641042E-412.6340233
CTATACA954.1641042E-412.6340234
ATTCTAT1006.413301E-412.002321
GTATACA950.005969962611.05476951
AGTAGAG950.005969962611.05476955
TATACCA950.005969962611.05476955
TTACCAG950.005969962611.05476955
TCAAGAC950.005969962611.05476953
ATAGTAC950.00598515111.0509988
ACTGTTC1100.00142893110.9074788
TTCTATA1253.398855E-410.8020892
ACAATAC1000.008738372510.502033
CAGTAGA1000.008738372510.502034
TCTATAC1150.002063504210.4368013
Produced by FastQC (version 0.11.2)