FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_32.35100000038e49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_32.35100000038e49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709138
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACC400.006508183718.74995423
GGACTGC550.002100608816.3635986
TGGGGGG1102.3535904E-816.3635985
GCCAGCC653.4361993E-416.152677
TAGGTAG1052.4740802E-715.7142495
CTCCTAA901.579889E-514.9999651
GTCCAGG600.003752090514.9999651
ACAGCGC600.003753851414.9989078
TATACTG1851.8189894E-1214.5945615
GTATTAG1156.9330235E-714.3477921
ATGACTG1054.2643023E-614.2856815
CTAATAC1802.1827873E-1114.1666343
AATACTA2250.013.9999685
CCACGAG650.006382965513.84612320
AGCGCAC650.006385947613.84514510
CTAGACC902.6497542E-413.33330254
CAGCGCA902.6513363E-413.3323629
ACTGGAC800.001655348213.1249693
TCTAAGC1151.0915692E-513.04344753
TAACACA1507.364724E-812.99996954
Produced by FastQC (version 0.11.2)