FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_31.35100000038e05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_31.35100000038e05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639044
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGAC957.943345E-817.36647811
TGGGGGG3100.015.0033575
CCGGACG1054.277388E-614.28411512
GTCCGCT851.6356686E-414.11606711
GTATTAG5300.013.0217821
TAGGTAG2650.012.4556175
TCTAGAC2051.9281288E-1012.4418083
TTAGGAC1702.820343E-812.3557053
GGGGGGC850.002615274412.3515598
TTGGGGG2750.012.0026864
CTAATAC4650.011.9381553
TACACTG2900.011.8992145
AGTCGGA1406.309192E-611.78727330
CACTGCG1151.5812789E-411.737467
GTATAGG3500.011.5740181
TATACTG6800.011.4731555
GTACTAG2659.094947E-1211.3232891
AGCGCAC1355.657068E-511.10986810
CGGACGG1355.657068E-511.10986813
GAGCGCA950.006003448711.0513959
Produced by FastQC (version 0.11.2)